The primary cell-based research and related potential application areas increased with the development of next-generation genome studies. The National Biobank of Korea launched a pilot project for collecting primary cells from human tissues to test the feasibility and usability of primary cells using a biobank.
This project was operated to secure human primary cells, including relevant information, and to establish the Standard Operating Procedure (SOP) of collecting, managing, storing and re-suspension. Common data elements for clinical/epidemiological information and data related to each of the primary cells were collected. Through this project, we successfully collected several primary cells (bone marrow-derived mesenchymal stem cells retrieved during bone marrow screening for the diagnostics of blood disease; bone marrow-derived stromal cells; mesenchymalcells, endothelial cells; and polyp-derived mesenchymal cells during endoscopic screening of large intestine disease; polyp stromal cells; polyp-derived mesenchymal cells, periodontal tissue-derived mesenchymal cells; oral fibroblast cells; fat-derived adipose stem cell from adipose tissue obtained during liposuction; keratinocytes; and mesenchymal cells and fibroblast cells from foreskin tissue obtained during circumcision).
Finally, we successfully obtained 11 cell types from 5 tissues. Also, the viability of re-suspended primary cells was checked. We concluded that these primary cells may be stored in LN2 tank and distributed for future-related studies.
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